rabbit anti cxcl16  (Bioss)

Journal: PLOS Pathogens

Article Title: The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney

doi: 10.1371/journal.ppat.1012969

Figure Lengend Snippet: (A) CXCL16 mRNA expression in the kidney during MuPyV infection. Expression is shown as fold change relative to sham infected samples. Data are from three independent experiments (n = 10-11). (B) Expression of NKCC2 [marks the ascending loop of Henle ], CD8, and CXCL16 in epithelia in sham-infected and 8 dpi kidneys; bottom right photomicrographs are merged images. Representative of two independent experiments. (C) CXCL16 expression in sham-infected, 4 dpi, and 8 dpi kidney lysates (left). Western blot image is representative of two independent experiments with each lane indicating protein lysate from kidneys of individual mice. Protein band intensity quantification for sCXCL16 was normalized to β-actin and analyzed by ImageLab and normalized to the loading control (right). Data are combined from two independent experiments (n = 3-5). (D) Experimental design of in vivo CXCL16 mAb administration. Mice were administered 250 µg of CXCL16 mAb or control rat IgG every two days from days 4-14 post infection and euthanized at 15 dpi. Numbers of CD45 mAb i.v.-negative, CD8 + CD44 + D b -LT359 tetramer + T cells and CD4 + CD44 + T cells in kidneys and spleens of infected mice given anti-CXCL16 or control rat IgG. Data were analyzed by one-way ANOVA (A and C) and by multiple Mann-Whitney tests (D).

Article Snippet: Membranes were incubated overnight at 4°C with rabbit anti-CXCL16 (Bioss) or rabbit anti-β-actin (Cell Signaling Technology) in 1% milk in TBS-T. Blots were washed with TBS-T followed by a 1.5 h incubation with horseradish peroxidase conjugated secondary antibodies (BioLegend) in 1% milk in TBS-T. After another wash with TBS-T, blots were developed with SuperSignal West Pico PLUS Chemiluminescence Substrate (ThermoFisher Scientific).

Techniques: Expressing, Infection, Western Blot, Control, In Vivo, MANN-WHITNEY

Journal: PLOS Pathogens

Article Title: The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney

doi: 10.1371/journal.ppat.1012969

Figure Lengend Snippet: (A) Dense lymphoplasmacytic infiltrate surrounding and infiltrating cortical tubules (H&E, x400). (B) Immunohistochemical staining for Large T antigen in nuclei of tubular epithelium (x250). (C) Expression of CXCR6 on infiltrating CD8 + cells. Photomicrographs (right) are enlarged images in the white squares (left); bottom right are merged images. No 1 o , no primary antibody. (D) Log-fold change of CXCL16 and CXCR6 across four independent studies, each comparing KTx biopsies from patients with PVAN and stable graft function. The error bars indicate the 95% confidence interval of log-fold change, with horizontal dashed grey line indicating log-fold change = 0, or no difference. GSE120495 is an RNA-seq study, while remaining studies are microarray based. Given the heterogeneity of the data, a Cauchy combination method with uniform weights was employed to integrate the p-values across these studies. The combined p-values for CXCL16 = 0.000337 and CXCR6 = 2.72x10 -05 . ns, non-significant at adjusted p-value > 0.05; *, statistical significance at adjusted p-value ≤ 0.05; **, statistical significance at adjusted p-value ≤ 0.01; ***, statistical significance at adjusted p-value ≤ 0.001.

Article Snippet: Membranes were incubated overnight at 4°C with rabbit anti-CXCL16 (Bioss) or rabbit anti-β-actin (Cell Signaling Technology) in 1% milk in TBS-T. Blots were washed with TBS-T followed by a 1.5 h incubation with horseradish peroxidase conjugated secondary antibodies (BioLegend) in 1% milk in TBS-T. After another wash with TBS-T, blots were developed with SuperSignal West Pico PLUS Chemiluminescence Substrate (ThermoFisher Scientific).

Techniques: Immunohistochemical staining, Staining, Expressing, RNA Sequencing, Microarray

Journal: PLOS Pathogens

Article Title: The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney

doi: 10.1371/journal.ppat.1012969

Figure Lengend Snippet: Summary of differential gene expression of CXCL16 and CXCR6 in four studies of KTx biopsies with PVAN. Because of the heterogeneity of the data (i.e., one RNA-seq, three microarray), a Cauchy combination method with uniform weights was employed to integrate the p-values across these studies. P-values significant after false discover rate (FDR) correction are bolded and italicized in the Adj. P-value column. Cauchy combined p-values < 0.05 are bolded and italicized.

Article Snippet: Membranes were incubated overnight at 4°C with rabbit anti-CXCL16 (Bioss) or rabbit anti-β-actin (Cell Signaling Technology) in 1% milk in TBS-T. Blots were washed with TBS-T followed by a 1.5 h incubation with horseradish peroxidase conjugated secondary antibodies (BioLegend) in 1% milk in TBS-T. After another wash with TBS-T, blots were developed with SuperSignal West Pico PLUS Chemiluminescence Substrate (ThermoFisher Scientific).

Techniques: Gene Expression, Microarray

Journal: Pharmaceutics

Article Title: Resveratrol Inhibited ADAM10 Mediated CXCL16-Cleavage and T-Cells Recruitment to Pancreatic β-Cells in Type 1 Diabetes Mellitus in Mice

doi: 10.3390/pharmaceutics14030594

Figure Lengend Snippet: ( A ) Double immunofluorescence staining for insulin (red), a pancreatic β cell marker, and CXCL16 (green) was performed; basal expression of pancreatic β cell CXCL16 in normal control and RES-treated mouse tissues. Increased CXCL16 expression in pancreatic β cells was confirmed by the co-localization of CXCL16 with insulin. Marked reduction of CXCL16 expression in mouse pancreatic β cells of RES-treated mice with STZ was seen; ( B ) Fluorescence intensity of CXCL16 protein expression for all groups was quantified and blotted; ( C ) Serum levels of CXCL16 using ELISA in all treated groups show similar findings of immunofluorescence. Data represent mean ± SEM: a difference is significant compared to control group; b difference is significant compared to RES group; c difference is significant compared to diabetic group at p < 0.05.

Article Snippet: Goat anti-rabbit polyclonal CXCL16 Ab was obtained from Peprotech (London, UK).

Techniques: Double Immunofluorescence Staining, Marker, Expressing, Control, Fluorescence, Enzyme-linked Immunosorbent Assay, Immunofluorescence